Mineral crystallization is central to myriad natural processes from the formation of snowflakes to stalagmites, but the molecularscale mechanisms are often far more complex than models reflect. Feedbacks between the hydro-, bio-, and geo-spheres drive complex crystallization processes that challenge our ability to observe and quantify them, motivating an expansion of crystallization theories. In this article, we discuss how the driving forces and timescales of nucleation are influenced by factors ranging from simple geometric confinement to distinct interfacial solution structures involving solvent organization, electrical double layers, and surface charging effects. Taken together, these ubiquitous natural phenomena can preserve metastable intermediates, drive precipitation of undersaturated phases, and modulate crystallization in time and space.
Language and language education are central to studies of Chinese diasporic culture. However, existing scholarship has overwhelmingly focused on how overseas Chinese populations navigate language politics in their host societies. This research adopts a different perspective by examining the crucial roles overseas Chinese played in establishing Indonesian language programs in mainland China between the mid-1940s and mid-1960s. Specifically, overseas Chinese “returnees” were indispensable in founding the National College of Oriental Studies during World War II and launching several Indonesian language programs in the early years of the People’s Republic of China. While these programs served vastly different political purposes over time, they also reveal critical yet often overlooked aspects of—and surprising continuities in—China-Indonesia cultural exchange amid decolonization, domestic conflicts, and the Cold War. Although the primary aim of these programs was to fulfill the operational needs of state agencies and government-affiliated organizations, returnee networks played essential roles in promoting Indonesian culture in China. They actively participated in circulatory cultural diplomacy between the two countries, contributing significantly to China’s long-term knowledge production on Indonesia.
Effective risk assessment and control of environmental antibiotic resistance depend on comprehensive information about antibiotic resistance genes (ARGs) and their microbial hosts. Advances in sequencing technologies and bioinformatics have enabled the identification of ARG hosts using metagenome-assembled contigs and genomes. However, these approaches often suffer from information loss and require extensive computational resources. Here we introduce a bioinformatic strategy that identifies ARG hosts by prescreening ARG-like reads (ALRs) directly from total metagenomic datasets. This ALR-based method offers several advantages: (1) it enables the detection of low-abundance ARG hosts with higher accuracy in complex environments; (2) it establishes a direct relationship between the abundance of ARGs and their hosts; and (3) it reduces computation time by approximately 44–96% compared to strategies relying on assembled contigs and genomes. We applied our ALR-based strategy alongside two traditional methods to investigate a typical human-impacted environment. The results were consistent across all methods, revealing that ARGs are predominantly carried by Gammaproteobacteria and Bacilli, and their distribution patterns may indicate the impact of wastewater discharge on coastal resistome. Our strategy provides rapid and accurate identification of antibiotic-resistant bacteria, offering valuable insights for the high-throughput surveillance of environmental antibiotic resistance. This study further expands our knowledge of ARG-related risk management in future.
Salt crystallization within micro-fractures poses a significant challenge in shale gas production by impeding gas diffusion. This study investigates the real-time behavior of gas flow-induced salt crystallization within a visualized micro-fracture network. Observations reveal that salt crystals initially propagate along the fracture surface before exhibiting perpendicular growth. Crystal nucleation during the saturation stage occurs within a few seconds, while subsequent growth in the supersaturated stage takes approximately 15–20 s. Gas flow drives the evaporation of immobile water, leading to salt precipitation. Furthermore, increasing gas flow rate and decreasing solution salinity are found to accelerate crystal growth. To mitigate plugging damage caused by salt crystallization, controlling pressure differences and solution salinity is crucial.
OBJECTIVES: Patients with polypoidal choroidal vasculopathy (PCV) exhibit variability in response to anti-VEGF therapy. This study aimed to analyse the aqueous humour proteomic profiles of PCV patients and provide preliminary insights for the identification of biomarkers associated with anti-VEGF drug responsiveness. METHODS: PCV patients who were treatment-naïve or untreated for more than 3 months were prospectively recruited from two hospitals in Beijing and Tianjin. Based on the relative changes in central macular thickness (ΔCMT/baseline-CMT) before and after anti-VEGF treatment, the PCV patients were divided into a good response (GR) group (≤-25%) and a poor response (PR) group (>-25%). Aqueous humour proteomics was performed by the Data-independent Acquisition-Mass Spectrometry (DIA-MS) method, and differentially expressed proteins (DEPs) analysis between the different PCV groups and the control group was conducted. Key DEPs were selected for preliminary validation in the aqueous humour using the Luminex method retrospectively. RESULTS: A total of 31 PCV patients (31 eyes) were included, 13 in the GR group and 18 in the PR group. A total of 414 DEPs were identified, including 36 significantly upregulated proteins, such as G protein regulatory factor 10 (RGS10), podocin (PODN) and epidermal growth factor (EGF), and 32 downregulated proteins, including RAB11FIP4 (Rab11 family-interacting protein 4), α-synuclein (SNCA), haemoglobin subunit δ (HBD) and interleukin 6 (IL6). Compared to the cataract control group (10 eyes), 134 proteins were significantly upregulated, and 72 were downregulated. KEGG pathway enrichment analysis revealed that the GR and PR groups differ in terms of cell communication, and cell signal transduction. Protein-protein interaction analysis revealed interactions between EGF and various DEPs. Validation of aqueous humour proteins using the Luminex method revealed that changes in the levels of EGF were associated with the anti-VEGF treatment response in PCV patients. CONCLUSIONS: PCV patients with good or poor anti-VEGF responses exhibit distinct aqueous humour proteomic profiles. Aqueous EGF may serve as a biomarker for the 'precise treatment' of PCV.
Abstract Vanadium dioxide (VO2), renowned for its reversible metal-to-insulator transition (MIT), has been widely used in configurable photonic and electronic devices. Precisely tailoring the MIT of VO2 on micro-/nano-scale is crucial for miniaturized and integrated devices. However, existing tailoring techniques like scanning probe microscopy, despite their precision, fall short in efficiency and adaptability, particularly on complex or curved surfaces. Herein, this work achieves the local engineering of the phase of VO2 films in high efficiency by employing laser writing to assist in the hydrogen doping or dedoping process. The laser doping and laser dedoping technique is also highly flexible, enabling the fabrication of reconfigurable, non-volatile, and multifunctional VO2 devices. This approach establishes a new paradigm for creating reconfigurable micro/nanophotonic and micro/nanoelectronic devices.
A few colonies in the Latin West (Augusta Emerita, Johnston 2017: 56-57 fn. 202-203; Abascal 2002: 284) and the Greek East (e.g. Philippi AE 1932, 21; briefly Samos IG XII,6 1:187 l. 8-11) used the annus coloniae, the year when the colony was founded, as their standard time-reckoning formula on inscriptions and perhaps other media as well. The list may include the Roman colony of Sinope, which coin issues carried legends such as C(olonia) F(elix) I(ulia) an(no) followed by (and later on simply with) Latin numerals (e.g. RPC I 2110, II 715). Curiously, since the 17th century, numismatists have observed two epochs on Sinope’s Roman period issues (e.g. Hardouin 1689: 140). There was the epoch of 45 BCE, likely the annus coloniae (e.g. RPC III 1229), as it coincided with reports of a Caesarian colony (Strab. 12.3.11). Then there was the epoch of 70 BCE (e.g. RPC VI 6501), seemingly replacing the earlier epoch, but only from the early third century onwards (Kubitschek 1908: 68-71; Magie 1950: 342 no. 42). Changing epochs was not a rare phenomenon in Anatolia, but known examples changed an earlier epoch for a later one, apparently to adapt to new circumstances, such as an imperial victory or visit (Leschhorn 1993: 439-541), and count years from a new beginning. Sinope, however, changed the epoch that was supposedly linked to the year of its colonial foundation to an earlier epoch that coincided with Lucullus’ “liberation” of Sinope from Mithridatid control. This suggests that Sinope was adapting to new circumstances that required the adaptation of an historical epoch. It is not the beginning per se, but a recaliberation of municipal history. While historical developments of Roman Sinope is not well documented in extant literary sources, and epigraphic evidence is scarce, this paper wishes to take two approaches to assess the problem of Sinope’s changing epochs. The first approach is to consider Sinope’s time-reckoning tradition. The era system that was prevalent in northern Anatolia and elsewhere during the first three centuries CE had a strong Hellenistic tradition. In this view, there may be more nuance – 45 BCE can be the annus coloniae, but it was reflecting a recent change in the city’s history, not an overhaul of the city’s established time-reckoning system, perhaps based on the Seleucid era (found on ceramic stamps, Saprykin & Fedoseev 1999: 135-143). This approach removes the Roman symbolism attached to the 45 BCE, and opens up additional possibilities for interpretation. The second approach considers the epoch of 70 BCE regionally. This epoch was primarily used at Amastris, but also attested at Abonuteichos. Both cities were described by Roman authors as prosperous in their own ways (e.g. Luc. Alex. 9, 25; Plin. Ep. 10.98). Both cities also began to issue 3 to 4 units of local bronze coinage from the reign of Trajan onwards, including the 6-assaria, associated with economic prosperity (Zajac 2023: 30-32, Tab. 1a). Epigraphic and numismatic evidence further suggest that Amastrians were mobile across the Black Sea and the Aegean, some even serving as koinon officials of Bithynian and other regional koina (Marek 2003: 63-67). There is the possibility that Sinope changed its epoch to adhere to regional time-reckoning norms set by more dominant peers. Since the 17th century, numismatists have observed two epochs on Sinope’s Roman period coin issues (e.g. Hardouin 1689: 140). There was the epoch of 45 BCE, perhaps the annus coloniae (e.g. RPC III 1229; cf. Augusta Emerita, Johnston 2017: 56-57 fn. 202-203; Abascal 2002: 284; Philippi AE 1932, 21; briefly Samos IG XII,6 1:187 l. 8-11), as it coincided with reports of a Caesarian colony (Strab. 12.3.11). Then there was the epoch of 70 BCE (e.g. RPC VI 6501), seemingly replacing the earlier epoch, but only from the early third century onwards (Kubitschek 1908: 68-71; Magie 1950: 342 no. 42). Neither changing epochs or the use of many epochs were rare phenomena. That said, known examples show cities replacing old epochs with those that commemorate new events and circumstances (Leschhorn 1993: 439-541). It is then odd for Sinope to abandon the epoch commemorating its colonial foundation in favor of an epoch 25 years earlier. Why? Leschhorn gave two potential scenarios: perhaps 70 BCE was the outcome a pro-Severan party defeating the old guard, or there was a “Gräzisierung" movement under which Sinope opted for a symbolically more acceptable epoch (Leschhorn 1993: 162), but these suggestions are limited by lack of evidence. This paper reassesses Sinope’s changing epochs with two approaches. First is a reframing of the Leschhorn’s assumed constitutional symbolism attached to the epoch of 45 BCE that leads to factionalism, and the “Roman” nature of Sinope’s so-called Kolonieära in Leschhorn’s alternative “Gräzisierung” thesis. The question asked here is whether Sinope had an established era system in place as part of its Hellenistic past. Sinope likely used the Pontic era while under Mithridatid control (Leschhorn 1993: 150). Recent discussions of stamped amphorae further indicates that the Seleucid era system was likely present if not also used at Sinope (Saprykin & Fedoseev 1999: 135-143). Further, we now have ceramic tiles time-stamped with a formula such as "s(aeculo) S(inopensis) (colonia) i(nducta) an(no) IV" (Fedoseev 2019: 16-17). If Fedoseev’s reading of the time-stamp is correct, we may consider the possibility that the saeculum Sinopensis was a system that could have accommodated multiple epochs, and the phenomenon in question was a symptom of an entrenched but adaptative time-reckoning mechanism at work. The second approach considers the epoch of 70 BCE regionally. This epoch was primarily used at Amastris, but also attested at Abonuteichos. Both cities were described by Roman authors as prosperous in their own ways (e.g. Luc. Alex. 9, 25; Plin. Ep. 10.98). Both cities also began to issue 3 to 4 units of local bronze coinage from the reign of Trajan onwards, including the 6-assaria, associated with economic prosperity (Zajac 2023: 30-32, Tab. 1a). Epigraphic and numismatic evidence further suggest that Amastrians were mobile across the Black Sea and the Aegean, some even serving as koinon officials of Bithynian and other regional koina (Marek 2003: 63-67). There is the possibility that Sinope changed its epoch to adhere to regional time-reckoning norms set by more dominant peers.
In response to the growing prevalence of online second language learning and the burgeoning field of international Chinese language education, this study examines the impact of multimodal inputs (MMI) on vocabulary acquisition within online environments among learners of Chinese as a second language (CSL). A teaching intervention was conducted with 90 Mongolian CSL learners, who were grouped into audiovisual, audio, and visual groups. The findings indicate that the audiovisual condition significantly improved vocabulary retention compared to the single-modality conditions in a delayed post-test. Nevertheless, the efficacy of the MMI treatment was observed to vary with learners’ proficiency levels, with beginner-level CSL learners deriving greater benefit from MMI than intermediate-level learners. Furthermore, participants expressed both favorable and critical perspectives regarding the application of MMI in vocabulary instruction. These results highlight the potential of MMI interventions to enhance vocabulary learning in online second-language education, while also underscoring the necessity of considering learners’ target language proficiency and their attitudes when developing MMI-based instructional approaches.
Awe, a self-transcendent emotion, has been theoretically posited as a precursor to wise reasoning. However, direct empirical evidence supporting this relationship and the underlying mechanism has been limited. In four studies (N = 3700), we examined the relationship between awe and wise reasoning, as well as the mediating effect of self-transcendence, employing cross-sectional, longitudinal, and experimental designs. We consistently found that awe had a lagged effect on (Study 1), enhanced (Studies 2 & 3), and was associated with (Study 4) wise reasoning. Furthermore, self-transcendence mediated this relationship (Studies 3 & 4). The impact of awe on wise reasoning and mediating effect of self-transcendence could not solely be attributed to awe’s predominantly positive nature, and the mediation model was established beyond the influence of self-smallness (Studies 3–4). These findings contribute to understanding the emotional trigger of wise reasoning, the cognitive implications of awe, and its role in promoting wise conflict resolution.