Effective risk assessment and control of environmental antibiotic resistance depend on comprehensive information about antibiotic resistance genes (ARGs) and their microbial hosts. Advances in sequencing technologies and bioinformatics have enabled the identification of ARG hosts using metagenome-assembled contigs and genomes. However, these approaches often suffer from information loss and require extensive computational resources. Here we introduce a bioinformatic strategy that identifies ARG hosts by prescreening ARG-like reads (ALRs) directly from total metagenomic datasets. This ALR-based method offers several advantages: (1) it enables the detection of low-abundance ARG hosts with higher accuracy in complex environments; (2) it establishes a direct relationship between the abundance of ARGs and their hosts; and (3) it reduces computation time by approximately 44–96% compared to strategies relying on assembled contigs and genomes. We applied our ALR-based strategy alongside two traditional methods to investigate a typical human-impacted environment. The results were consistent across all methods, revealing that ARGs are predominantly carried by Gammaproteobacteria and Bacilli, and their distribution patterns may indicate the impact of wastewater discharge on coastal resistome. Our strategy provides rapid and accurate identification of antibiotic-resistant bacteria, offering valuable insights for the high-throughput surveillance of environmental antibiotic resistance. This study further expands our knowledge of ARG-related risk management in future.
Salt crystallization within micro-fractures poses a significant challenge in shale gas production by impeding gas diffusion. This study investigates the real-time behavior of gas flow-induced salt crystallization within a visualized micro-fracture network. Observations reveal that salt crystals initially propagate along the fracture surface before exhibiting perpendicular growth. Crystal nucleation during the saturation stage occurs within a few seconds, while subsequent growth in the supersaturated stage takes approximately 15–20 s. Gas flow drives the evaporation of immobile water, leading to salt precipitation. Furthermore, increasing gas flow rate and decreasing solution salinity are found to accelerate crystal growth. To mitigate plugging damage caused by salt crystallization, controlling pressure differences and solution salinity is crucial.
Despite its critical role in regulating the global climate and carbon cycle, the evolution of deep Pacific circulation has not been fully deciphered during the last glacial cycle. The effect of deep Pacific hydrographic change (e.g. oxygenation and circulation) on atmospheric CO2 variation is still uncertain. Here, we study redox-sensitive elements including V-U-Mn and benthic foraminiferal δ13C at the HYIV2015-B9 site in the southern South China Sea (SCS) to reconstruct the oxygenation and δ13C signals of water masses during the last glacial cycle. The intra-basin benthic foraminiferal δ13C gradient suggests enhanced stratification of the deep Pacific during the glacial compared to the interglacial, implying sluggish abyssal Pacific overturning. This is consistent with weak Pacific Deep Water (PDW) ventilation, as indicated by high contents of authigenic V and U, and low authigenic Mn. The inferred sluggish abyssal Pacific overturning is probably associated with less transport of Lower Circumpolar Deep Water, facilitating the expansion of respired carbon storage in the glacial deep Pacific. Meanwhile, the atmospheric CO2 rise is closely related to active abyssal Pacific overturning since late MIS 5, particularly when considering the impact of Southern Ocean upwelling modulated by Earth's obliquity. Overall, our data indicate the critical role of abyssal Pacific overturning in the carbon cycle, revealing the potential pathway for deep carbon dioxide outgassing in the North Pacific.
OBJECTIVES: Patients with polypoidal choroidal vasculopathy (PCV) exhibit variability in response to anti-VEGF therapy. This study aimed to analyse the aqueous humour proteomic profiles of PCV patients and provide preliminary insights for the identification of biomarkers associated with anti-VEGF drug responsiveness. METHODS: PCV patients who were treatment-naïve or untreated for more than 3 months were prospectively recruited from two hospitals in Beijing and Tianjin. Based on the relative changes in central macular thickness (ΔCMT/baseline-CMT) before and after anti-VEGF treatment, the PCV patients were divided into a good response (GR) group (≤-25%) and a poor response (PR) group (>-25%). Aqueous humour proteomics was performed by the Data-independent Acquisition-Mass Spectrometry (DIA-MS) method, and differentially expressed proteins (DEPs) analysis between the different PCV groups and the control group was conducted. Key DEPs were selected for preliminary validation in the aqueous humour using the Luminex method retrospectively. RESULTS: A total of 31 PCV patients (31 eyes) were included, 13 in the GR group and 18 in the PR group. A total of 414 DEPs were identified, including 36 significantly upregulated proteins, such as G protein regulatory factor 10 (RGS10), podocin (PODN) and epidermal growth factor (EGF), and 32 downregulated proteins, including RAB11FIP4 (Rab11 family-interacting protein 4), α-synuclein (SNCA), haemoglobin subunit δ (HBD) and interleukin 6 (IL6). Compared to the cataract control group (10 eyes), 134 proteins were significantly upregulated, and 72 were downregulated. KEGG pathway enrichment analysis revealed that the GR and PR groups differ in terms of cell communication, and cell signal transduction. Protein-protein interaction analysis revealed interactions between EGF and various DEPs. Validation of aqueous humour proteins using the Luminex method revealed that changes in the levels of EGF were associated with the anti-VEGF treatment response in PCV patients. CONCLUSIONS: PCV patients with good or poor anti-VEGF responses exhibit distinct aqueous humour proteomic profiles. Aqueous EGF may serve as a biomarker for the 'precise treatment' of PCV.
Abstract Vanadium dioxide (VO2), renowned for its reversible metal-to-insulator transition (MIT), has been widely used in configurable photonic and electronic devices. Precisely tailoring the MIT of VO2 on micro-/nano-scale is crucial for miniaturized and integrated devices. However, existing tailoring techniques like scanning probe microscopy, despite their precision, fall short in efficiency and adaptability, particularly on complex or curved surfaces. Herein, this work achieves the local engineering of the phase of VO2 films in high efficiency by employing laser writing to assist in the hydrogen doping or dedoping process. The laser doping and laser dedoping technique is also highly flexible, enabling the fabrication of reconfigurable, non-volatile, and multifunctional VO2 devices. This approach establishes a new paradigm for creating reconfigurable micro/nanophotonic and micro/nanoelectronic devices.
In response to the growing prevalence of online second language learning and the burgeoning field of international Chinese language education, this study examines the impact of multimodal inputs (MMI) on vocabulary acquisition within online environments among learners of Chinese as a second language (CSL). A teaching intervention was conducted with 90 Mongolian CSL learners, who were grouped into audiovisual, audio, and visual groups. The findings indicate that the audiovisual condition significantly improved vocabulary retention compared to the single-modality conditions in a delayed post-test. Nevertheless, the efficacy of the MMI treatment was observed to vary with learners’ proficiency levels, with beginner-level CSL learners deriving greater benefit from MMI than intermediate-level learners. Furthermore, participants expressed both favorable and critical perspectives regarding the application of MMI in vocabulary instruction. These results highlight the potential of MMI interventions to enhance vocabulary learning in online second-language education, while also underscoring the necessity of considering learners’ target language proficiency and their attitudes when developing MMI-based instructional approaches.
Awe, a self-transcendent emotion, has been theoretically posited as a precursor to wise reasoning. However, direct empirical evidence supporting this relationship and the underlying mechanism has been limited. In four studies (N = 3700), we examined the relationship between awe and wise reasoning, as well as the mediating effect of self-transcendence, employing cross-sectional, longitudinal, and experimental designs. We consistently found that awe had a lagged effect on (Study 1), enhanced (Studies 2 & 3), and was associated with (Study 4) wise reasoning. Furthermore, self-transcendence mediated this relationship (Studies 3 & 4). The impact of awe on wise reasoning and mediating effect of self-transcendence could not solely be attributed to awe’s predominantly positive nature, and the mediation model was established beyond the influence of self-smallness (Studies 3–4). These findings contribute to understanding the emotional trigger of wise reasoning, the cognitive implications of awe, and its role in promoting wise conflict resolution.
This study aims to identify the associations between teacher mental health and student mental health. Cross-sectional data were collected from 127,877 students aged 9–20 years and 2,759 teachers across 31 provinces in China. The mental health of students and teachers were assessed by well-being (life satisfaction and positive mental health), and psychological distress (depression and anxiety). Controlling for demographic variables, multilevel regression analyses suggest that higher teacher positive mental health was linked to higher student positive mental health and lower student depression; higher teacher depression were correlated with higher student depression; and teacher life satisfaction and anxiety were not correlated with any indicators of student mental health. The study highlights the significant association between teacher mental health and student mental health.