Freeze–thaw (FT) cycle, a ubiquitous and important physical process in natural environments, may influence the horizontal gene transfer (HGT) process of antibiotic-resistant genes (ARGs), yet its impact and underlying mechanisms remain unclear. This study investigated the impact and mechanisms of FT cycles on ARG conjugative transfer among intra- and interspecies under different solution ionic strength conditions. The results showed that despite ionic strengths and species pairs, different FT treatment cycles induced diverse influences on ARG conjugation frequency with inhibition effect of 1 FT cycle (decreased by 3.5–76-fold) and facilitation effect of 2 and 3 FT cycles (promoted by 2–27-fold). The reduced conjugation frequency after 1 FT treatment cycle is mainly attributed to energy deficiency caused by the starvation process during FT treatment. The enhanced cell–cell adhesion induced by the increased hydrophobicity and secretion of bacterial extracellular polymeric substances (EPS) facilitated the formation of conjugative pilus, primarily promoting the ARG conjugative transfer process after 2 and 3 FT treatment cycles. The results of our study clearly show that FT cycles (intensified by the ongoing climate change) exhibit profound influence on the dissemination of ARGs, which helps understand and predict ARG risks in different natural environments.
This paper revisits the commercial peace hypothesis using global event data from 1989 to 2019. We show that greater imports consistently worsen bilateral relations, with the effect strengthening after the 2008 financial crisis. The negative impact is most pronounced in democracies, high-income countries and those with elevated unemployment, where globalisation's distributive conflicts more easily shape foreign policy. Instrumental variable estimates confirm causality, and analyses using militarised interstate disputes reveal that trade-driven tensions extend beyond diplomacy to overt conflict. These findings challenge the optimistic view that economic interdependence fosters peace.
Individualized head-related transfer functions (HRTFs) require accurate pinna geometry, yet commodity multi-view captures leave the ear region self-occluded and weakly textured. We present a practical pipeline that couples ear-centric acquisition with 3D Gaussian splatting (3DGS) and the boundary element method (BEM) for complete HRTF computation. The protocol augments horizontal views with per-ear elevated captures under directional lighting; 3DGS training with depth-distortion regularization yields watertight meshes via truncated signed distance function (TSDF) fusion. Standardized head coordinates and ear-canal annotations interface the mesh with BEM. Experimental evaluations demonstrate that our method achieves lower ear-region geometric error and lowerfull-band spectral distortion compared to existing image-based personalized reconstruction baselines including AudioEar, NeuS, and Metashape MVS.
Gender ideology can affect household energy consumption; however, the existing literature has ignored this aspect. Using data from household surveys, this study employs econometric modeling to examine the impact of gender ideology on household cooking fuel choices and their underlying mechanisms. The results show that an emancipatory gender ideology can substantially promote adoption of clean cooking fuels by households. The results of the mechanistic analysis show that gender ideology encourages the adoption of clean cooking fuels through two pathways: increased internet use and exercise. Furthermore, the impact of gender ideology on households' choice of cooking fuel is moderated by income and the importance of internet, with neighborhood spillover effects and heterogeneity. The HOUSE model, developed by integrating households and neighbors, clarifies the complex relationship between gender ideology and household cooking fuel choice. This study provides meaningful theoretical and practical guidance for encouraging rural households to adopt cleaner cooking fuel.
Hydroxymethyl hydroperoxide (HMHP, HOCH2OOH) is one of the most abundant organic peroxides (POs) in the atmosphere. Owing to its extremely high solubility, HMHP readily partitions into cloudwater and aerosol liquid water, where it hydrolyzes to hydrogen peroxide (H2O2) and formaldehyde (HCHO). However, previous studies were conducted in dilute solutions and did not adequately account for the high-salinity characteristic of deliquesced aerosol particles. Here, we systematically investigate the combined effects of pH (0–6), temperature (277–313 K), ionic strength (0–10 M), and ion identity (NH4+, Na+, SO42–, and Cl–) on the hydrolysis kinetics of HMHP. For the first time, a parametrization formula describing the dependence of the hydrolysis rate constant on ionic strength is established, demonstrating that ionic strength exerts only a limited influence on HMHP hydrolysis. However, it is found that in highly concentrated ammonium salt solutions, HMHP undergoes a previously unrecognized NH3-driven reaction pathway. This new pathway competes with hydrolysis, accelerating the apparent transformation rate of HMHP by more than an order of magnitude while significantly reducing the yield of H2O2 and HCHO. Our findings highlight that future atmospheric chemical models should fully account for the NH3-driven pathway in aqueous-phase reactions of POs, thereby enabling a more accurate assessment of the role of POs in atmospheric oxidant cycling and secondary particulate matter formation.
Formic acid and acetic acid are the most abundant gas-phase organic acids in the atmosphere, yet their concentrations are substantially underestimated by both global and regional atmospheric models across diverse environments. In this study, we report unexpectedly high yields of formic acid and acetic acid during the multi-generational photooxidation of toluene, a canonical anthropogenic volatile organic compound. Their yields exhibit a strong dependence on hydroxyl radical (•OH) exposure ([•OH] × residence time), increasing from 25 % and 24 % under low exposure (< 0.2 equivalent days) to 74 % and 40 % under elevated exposure (1–3 equivalent days) for formic and acetic acid, respectively. The formation of these organic acids is not significantly affected by NOx concentrations. A modified box model based on MCM v3.3.1 underestimates the peak concentrations of both acids by approximately a factor of five, indicating substantial gaps in current mechanistic understanding. Although both secondary aerosol formation and organic acid production increase with aging to a certain degree of oxidation, their distinct temporal evolutions suggest that particle photodegradation is not the dominant pathway. The contrasting •OH exposure dependence between organic acids and primary carbonyl compounds further implies that these acids are predominantly multi-generational oxidation products. These findings demonstrate that multi-generational oxidation of aromatic compounds is an important and previously underappreciated source of atmospheric organic acids. The omission of organic acid formation from aromatic oxidation in current chemical mechanisms likely contributes to their widespread underestimation in models, highlighting the need for detailed laboratory studies and updated chemical mechanisms.
