摘要:

ABSTRACT: A higher denitrification rate was realized via controlling the mass ratio of pyrite and poly(3-hydroxybutyrate-hydroxyvalerate) (PHBV) under natural aerobic conditions. The results showed that the suitable mass ratio of PHBV and pyrite could be 1:2 with its removal efficiency of nitrogen and phosphorus of 99.7 and 53.4%, respectively. The PHBV/pyrite system has formed the spatial patterns of the biofilm community, such as Dechloromons attached to the pyrite surface, Rhodocyclaceae attached to the PHBV surface, and Acidovorax attached to the suppled sludge, which highlighted that the autotrophic??? heterotrophic synergy was achieved. The difference analysis among functional genes detected by high-throughput quantitative polymerase chain reaction indicated that the surface of pyrite in the pyrite/PHBV system is the hot area of methane production, the denitrifying process, and phosphorus removal. Network analysis indicated that there was a closer connection among functional genes on the pyrite surface, also supporting the speculation that pyrite was the hot area for the interaction of various genes in the pyrite/PHBV system. The key gene co-occurrence revealed that lig, nirS, and aspA are the keystone genes for cellulose degradation, denitrification, and S cycling, respectively. These results suggested that the pyrite surface was the hot area for denitrification, phosphorus removal in the blending system with pyrite and PHBV for nitrogen and phosphorus removal.