摘要:

53#,a strain of efficient alginate degrading bacteria,is isolated from rotted kelp using sodium alginate as the only carbon resource. Strain 53# is identified as Paenibacillus agaridevorans based on physiological characteristics and 16S rRNA sequencing. The optimaltion yield condition of strain 53# is identified at ${\rm{pH = 8}}$,$T = 25^\circ {\rm{C}}$,NaCl 15 g/L,and sodium alginate 15 g/L by orthogonal experiment and analysis,and the highest enzyme activity is ${\rm{390}}{\rm{.53}} \pm {\rm{17}}{\rm{.32\;U/mL}}$. Strain 53# has the advantages such as being cultivated easily,producing enzyme fast and having high enzyme activity. It can achieve a high efficiency for saccharification of alginate and thus has potential value to be utilized in the production of bioethanol from brown algae.以海藻酸钠为唯一碳源,从天然腐烂海带中筛选得到一株高效褐藻胶降解菌株53#,经形态学观察和16S rRNA鉴定,确定为类芽孢杆菌Paenibacillus agaridevorans。采用正交试验方法,以pH、温度、NaCl浓度和海藻酸钠初始浓度为影响因素,对该菌株的产酶条件进行优化,得到53#菌的 最佳产酶条件: ${\rm{pH = 8}}$,25℃,NaCl浓度15 g/L,海藻酸钠初始浓度15 g/L。在最佳产酶条件下,褐藻胶裂解酶最大酶活可达${\rm{390}}{\rm{.53}} \pm {\rm{17}}{\rm{.32\;U/mL}}$。筛选得到的类芽孢杆菌Paenibacillus agaridevorans 53#具有易于培养、产酶速度快和酶活力高等优点,能够实现褐藻胶的高效糖化,在褐藻生产生物乙醇领域具有潜在利用价值。

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