<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tingting Li</style></author><author><style face="normal" font="default" size="100%">Qian Chen</style></author><author><style face="normal" font="default" size="100%">Shi, Ping</style></author><author><style face="normal" font="default" size="100%">Geng, Shu</style></author></authors><translated-authors><author><style face="normal" font="default" size="100%">李婷婷,</style></author><author><style face="normal" font="default" size="100%">陈倩,</style></author><author><style face="normal" font="default" size="100%">石萍,</style></author><author><style face="normal" font="default" size="100%">耿旭,</style></author></translated-authors></contributors><titles><title><style face="normal" font="default" size="100%">Screening of Alginate Degrading Bacteria and Optimization of Its Enzyme-Producing Conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Scientiarum Naturalium Universitatis PekinensisActa Scientiarum Naturalium Universitatis Pekinensis</style></secondary-title><translated-title><style face="normal" font="default" size="100%">高效褐藻胶降解菌的筛选与产酶条件优化</style></translated-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2017</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">6</style></number><volume><style face="normal" font="default" size="100%">53</style></volume><pages><style face="normal" font="default" size="100%">1115-1121</style></pages><isbn><style face="normal" font="default" size="100%">0479-8023</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">53#,a strain of efficient alginate degrading bacteria,is isolated from rotted kelp using sodium alginate as the only carbon resource. Strain 53# is identified as Paenibacillus agaridevorans based on physiological characteristics and 16S rRNA sequencing. The optimaltion yield condition of strain 53# is identified at ${\rm{pH = 8}}$,$T = 25^\circ {\rm{C}}$,NaCl 15 g/L,and sodium alginate 15 g/L by orthogonal experiment and analysis,and the highest enzyme activity is ${\rm{390}}{\rm{.53}} \pm {\rm{17}}{\rm{.32\;U/mL}}$. Strain 53# has the advantages such as being cultivated easily,producing enzyme fast and having high enzyme activity. It can achieve a high efficiency for saccharification of alginate and thus has potential value to be utilized in the production of bioethanol from brown algae.以海藻酸钠为唯一碳源,从天然腐烂海带中筛选得到一株高效褐藻胶降解菌株53#,经形态学观察和16S rRNA鉴定,确定为类芽孢杆菌Paenibacillus agaridevorans。采用正交试验方法,以pH、温度、NaCl浓度和海藻酸钠初始浓度为影响因素,对该菌株的产酶条件进行优化,得到53#菌的 最佳产酶条件: ${\rm{pH = 8}}$,25℃,NaCl浓度15 g/L,海藻酸钠初始浓度15 g/L。在最佳产酶条件下,褐藻胶裂解酶最大酶活可达${\rm{390}}{\rm{.53}} \pm {\rm{17}}{\rm{.32\;U/mL}}$。筛选得到的类芽孢杆菌Paenibacillus agaridevorans 53#具有易于培养、产酶速度快和酶活力高等优点,能够实现褐藻胶的高效糖化,在褐藻生产生物乙醇领域具有潜在利用价值。</style></abstract><accession-num><style face="normal" font="default" size="100%">CSCD:6123103</style></accession-num><notes><style face="normal" font="default" size="100%">Times Cited: 0</style></notes><custom7><style face="normal" font="default" size="100%">0479-8023(2017)53:6&amp;lt;1115:Gxhzjj&amp;gt;2.0.Tx;2-m</style></custom7></record></records></xml>