@article {31009,
	title = {Screening of Alginate Degrading Bacteria and Optimization of Its Enzyme-Producing Conditions},
	journal = {Acta Scientiarum Naturalium Universitatis PekinensisActa Scientiarum Naturalium Universitatis Pekinensis},
	volume = {53},
	number = {6},
	year = {2017},
	note = {Times Cited: 0},
	month = {2017},
	pages = {1115-1121},
	abstract = {53$\#$,a strain of efficient alginate degrading bacteria,is isolated from rotted kelp using sodium alginate as the only carbon resource. Strain 53$\#$ is identified as Paenibacillus agaridevorans based on physiological characteristics and 16S rRNA sequencing. The optimaltion yield condition of strain 53$\#$ is identified at ${\rm{pH = 8}}$,$T = 25^\circ {\rm{C}}$,NaCl 15 g/L,and sodium alginate 15 g/L by orthogonal experiment and analysis,and the highest enzyme activity is ${\rm{390}}{\rm{.53}} \pm {\rm{17}}{\rm{.32\;U/mL}}$. Strain 53$\#$ has the advantages such as being cultivated easily,producing enzyme fast and having high enzyme activity. It can achieve a high efficiency for saccharification of alginate and thus has potential value to be utilized in the production of bioethanol from brown algae.以海藻酸钠为唯一碳源,从天然腐烂海带中筛选得到一株高效褐藻胶降解菌株53$\#$,经形态学观察和16S rRNA鉴定,确定为类芽孢杆菌Paenibacillus agaridevorans。采用正交试验方法,以pH、温度、NaCl浓度和海藻酸钠初始浓度为影响因素,对该菌株的产酶条件进行优化,得到53$\#$菌的 最佳产酶条件: ${\rm{pH = 8}}$,25{\textcelsius},NaCl浓度15 g/L,海藻酸钠初始浓度15 g/L。在最佳产酶条件下,褐藻胶裂解酶最大酶活可达${\rm{390}}{\rm{.53}} \pm {\rm{17}}{\rm{.32\;U/mL}}$。筛选得到的类芽孢杆菌Paenibacillus agaridevorans 53$\#$具有易于培养、产酶速度快和酶活力高等优点,能够实现褐藻胶的高效糖化,在褐藻生产生物乙醇领域具有潜在利用价值。},
	isbn = {0479-8023},
	author = {Tingting Li and Qian Chen and Shi, Ping and Geng, Shu}
}
