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科研成果

2023
Yang J, Xiang N, Liu Y, Guo C, Li C, Li H, Cai S, Dixon R, Wang Y-P. Organelle-dependent polyprotein designs enable stoichiometric expression of nitrogen fixation components targeted to mitochondria. Proceedings of the National Academy of Sciences [Internet]. 2023;120(34):e2305142120. 访问链接
2020
Xiang N, Guo C, Liu J, Xu H, Dixon R, Yang J, Wang Y-P. Using synthetic biology to overcome barriers to stable expression of nitrogenase in eukaryotic organelles. Proceedings of the National Academy of Sciences [Internet]. 2020;117(28):16537-16545. 访问链接
2019
Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang Y-P, Jin Q, et al. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell [Internet]. 2019;177:370-383.e15. 访问链接Abstract
Summary Contractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of extracellular CISs that are present in both bacteria and archaea. Here, we report the cryo-EM structure of an intact PVC from P. asymbiotica. This over 10-MDa device resembles a simplified T4 phage tail, containing a hexagonal baseplate complex with six fibers and a capped 117-nanometer sheath-tube trunk. One distinct feature of the PVC is the presence of three variants for both tube and sheath proteins, indicating a functional specialization of them during evolution. The terminal hexameric cap docks onto the topmost layer of the inner tube and locks the outer sheath in pre-contraction state with six stretching arms. Our results on the PVC provide a framework for understanding the general mechanism of widespread CISs and pave the way for using them as delivery tools in biological or therapeutic applications.
2018
Yang J, Xie X, Xiang N, Tian Z-X, Dixon R, Wang Y-P. Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology. Proceedings of the National Academy of Sciences [Internet]. 2018;115(36):E8509-E8517. 访问链接
2017
Yang J, Xie X, Yang M, Dixon R, Wang Y-P. Modular electron-transport chains from eukaryotic organelles function to support nitrogenase activity. Proceedings of the National Academy of Sciences [Internet]. 2017;114(12):E2460-E2465. 访问链接
2014
Yang J, Xie X, Wang X, Dixon R, Wang Y-P. Reconstruction and minimal gene requirements for the alternative iron-only nitrogenase in Escherichia coli. Proceedings of the National Academy of Sciences [Internet]. 2014;111(35):E3718-E3725. 访问链接