The collapse of the former Soviet Union signaled failure of large-scale experiment in communitarian property. Privatization reform consequently was taken as the start point to transfer the planned economy to a market economy by the post socialist countries. This also occurred in economic transition countries such as China. However, in overcoming the tragedy of the commons privatization might create anticommons problems. Here we develop a nested common-private interface framework from the perspective of resource system and resource units and apply this framework to explain reforms of rangeland property in China and Kyrgyzstan. We confirmed that the root of the dilemma, either caused by commons or anticommons, can be attributed to the interface mismatch between individual elements and common elements. Trying to overcome the dilemma by changing property arrangements alone cannot eliminate the incentive mismatch caused by the common-private interface. Institutions aimed at alleviating the mismatch are accordingly required. Theoretically, this framework converts Ostrom's concept of commons into liberal commons that the members have options to exit, which is becoming increasingly common in the current global context of marketization. In the real world, this framework can serve to understand the property reform progress of transition countries, and may enlighten future property reforms.
Biomolecule labeling in living systems is crucial for understanding biological processes and discovering therapeutic targets. A variety of labeling warheads have been developed for multiple biological applications, including proteomics, bioimaging, sequencing, and drug development. Quinone methides (QMs), a class of highly reactive Michael receptors, have recently emerged as prominent warheads for on-demand biomolecule labeling. Their highly flexible functionality and tunability allow for diverse biological applications, but remain poorly explored at present. In this regard, we designed, synthesized, and evaluated a series of new QM probes with a trifluoromethyl group at the benzyl position and substituents on the aromatic ring to manipulate their chemical properties for biomolecule labeling. The engineered QM warhead efficiently labeled proteins both in vitro and under living cell conditions, with significantly enhanced activity compared to previous QM warheads. We further analyzed the labeling efficacy with the assistance of density functional theory (DFT) calculations, which revealed that the QM generation process, rather than the reactivity of QM, contributes more predominantly to the labeling efficacy. Noteworthy, twelve nucleophilic residues on the BSA were labeled by the probe, including Cys, Asp, Glu, His, Lys, Asn, Gln, Arg, Ser, Thr, Trp and Tyr. Given their high efficiency and tunability, these new QM warheads may hold great promise for a broad range of applications, especially spatiotemporal proteomic profiling for in-depth biological studies.