<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wang, D.</style></author><author><style face="normal" font="default" size="100%">Hou, Z.</style></author><author><style face="normal" font="default" size="100%">Q. Zhang</style></author><author><style face="normal" font="default" size="100%">Y. Zhou</style></author><author><style face="normal" font="default" size="100%">Lu, X.-X.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Impact of the Biodegradation of 6:2 Fluorotelomer Alcohol on the Bacterial Community Structure of Surface Sediment</style></title><secondary-title><style face="normal" font="default" size="100%">Huanjing Kexue/Environmental Science</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">alcohol</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacterial community structure</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacteriology</style></keyword><keyword><style  face="normal" font="default" size="100%">bacterium</style></keyword><keyword><style  face="normal" font="default" size="100%">biodegradation</style></keyword><keyword><style  face="normal" font="default" size="100%">bioremediation</style></keyword><keyword><style  face="normal" font="default" size="100%">Carboxylic acids</style></keyword><keyword><style  face="normal" font="default" size="100%">China</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">classification</style></keyword><keyword><style  face="normal" font="default" size="100%">Consumer products</style></keyword><keyword><style  face="normal" font="default" size="100%">Degradation</style></keyword><keyword><style  face="normal" font="default" size="100%">Denaturing gradient gel electrophoreses (DGGE)</style></keyword><keyword><style  face="normal" font="default" size="100%">Diversity index</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">ENVIRONMENTAL</style></keyword><keyword><style  face="normal" font="default" size="100%">Ethanol</style></keyword><keyword><style  face="normal" font="default" size="100%">fluorocarbon</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorocarbons</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorotelomer alcohols</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorotelomer carboxylic acids</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorotelomer unsaturated carboxylic acids</style></keyword><keyword><style  face="normal" font="default" size="100%">Geologic Sediments</style></keyword><keyword><style  face="normal" font="default" size="100%">High-throughput sequencing</style></keyword><keyword><style  face="normal" font="default" size="100%">Ketones</style></keyword><keyword><style  face="normal" font="default" size="100%">Liquid</style></keyword><keyword><style  face="normal" font="default" size="100%">Liquid chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">microbiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Microcosm experiments</style></keyword><keyword><style  face="normal" font="default" size="100%">river</style></keyword><keyword><style  face="normal" font="default" size="100%">Rivers</style></keyword><keyword><style  face="normal" font="default" size="100%">Sediment</style></keyword><keyword><style  face="normal" font="default" size="100%">sediments</style></keyword><keyword><style  face="normal" font="default" size="100%">Surficial sediments</style></keyword><keyword><style  face="normal" font="default" size="100%">Tandem mass spectrometry</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045697955&amp;amp;doi=10.13227%2fj.hjkx.201704297&amp;amp;partnerID=40&amp;amp;md5=59cdce8c3cd9b9b331248826e38e4422</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">11</style></number><publisher><style face="normal" font="default" size="100%">Science Press</style></publisher><volume><style face="normal" font="default" size="100%">38</style></volume><pages><style face="normal" font="default" size="100%">4747-4755</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Fluorotelomer alcohol (6:2 FTOH) is a polyfluoalkyl substance that has been widely used in industry and consumer products in recent years, causing potential harm to the environment. However, currently the impact of 6:2 FTOH and its degradation products on microbial communities in sediment is unclear. The purpose of this study is to explore the impact of the biodegradation of 6:2 FTOH on bacterial community structures in surface sediment based on gene analysis. Surface sediment and river water were collected from Hai river, Tianjin, and a microcosm experiment was performed in the laboratory. The concentration of 6:2 FTOH and its degradation products were analyzed by liquid chromatograph-mass spectrometry/mass spectrometry (LC-MS/MS). The bacterial community structure was analyzed by denaturing gradient gel electrophoresis and high-throughput sequencing. The results showed that 6:2 FTOH could be degraded by microorganisms (half-life was less than 3 d), producing transient products such as 6:2 fluorotelomer carboxylic acid (FTCA) and 6:2 fluorotelomer unsaturated carboxylic acid (FTUCA) and stable products such as 5:2 fluorotelomer (FT) ketone, 5:2 fluorotelomer alcohol (sFTOH), perfluorohexanoic acid(PFHxA), perfluoro-n-pentanoic acid (PFPeA), perfluorobutanoic acid (PFBA) and 5:3 polyfluorinated acid. At different stages of 6:2 FTOH degradation, a change of bacteria and the predominant population became somewhat different. Based on the experimental results for 100 d, at the Phylum level, the biodegradation of 6:2 FTOH greatly increases the abundance of Chloroflexi (+24.8%) and decreases the abundance of Proteobacteria (-17.8%) and Firmicutes (-15.9%). At the Class level, due to the biodegradation of 6:2 FTOH, bacteria with notable increases included Anaerolineae (+19.6%) and δ-Proteobacteria (+4.3%), while bacteria with notable decreases included ε-Proteobacteria (-20.0%), Clostridia (-10.1%), Bacilli (-5.8%) and γ-Proteobacteria (-4.2%). At the Genus level, due to the biodegradation of 6:2 FTOH, bacteria with notable increases included Anaerolineaceae_(uncultured) (+19.1%) and Thioalkalispira (+13.3%), while bacteria with notable decreases included Vibrio (-14.1%), Sulfurimonas (-13.2%), Bacillus (-5.1%), Sulfurovum (-4.2%) and Fusibacter (-4.1%). These results are helpful for predicting the response of bacteria to the contamination of polyfluoalkyl substances and isolating the bacteria capable of the biodegradation of polyfluoalkyl substances. © 2017, Science Press. All right reserved.</style></abstract><notes><style face="normal" font="default" size="100%">cited By 0</style></notes></record></records></xml>