@article {Wang20174747,
	title = {Impact of the Biodegradation of 6:2 Fluorotelomer Alcohol on the Bacterial Community Structure of Surface Sediment},
	journal = {Huanjing Kexue/Environmental Science},
	volume = {38},
	number = {11},
	year = {2017},
	note = {cited By 0},
	pages = {4747-4755},
	publisher = {Science Press},
	abstract = {Fluorotelomer alcohol (6:2 FTOH) is a polyfluoalkyl substance that has been widely used in industry and consumer products in recent years, causing potential harm to the environment. However, currently the impact of 6:2 FTOH and its degradation products on microbial communities in sediment is unclear. The purpose of this study is to explore the impact of the biodegradation of 6:2 FTOH on bacterial community structures in surface sediment based on gene analysis. Surface sediment and river water were collected from Hai river, Tianjin, and a microcosm experiment was performed in the laboratory. The concentration of 6:2 FTOH and its degradation products were analyzed by liquid chromatograph-mass spectrometry/mass spectrometry (LC-MS/MS). The bacterial community structure was analyzed by denaturing gradient gel electrophoresis and high-throughput sequencing. The results showed that 6:2 FTOH could be degraded by microorganisms (half-life was less than 3 d), producing transient products such as 6:2 fluorotelomer carboxylic acid (FTCA) and 6:2 fluorotelomer unsaturated carboxylic acid (FTUCA) and stable products such as 5:2 fluorotelomer (FT) ketone, 5:2 fluorotelomer alcohol (sFTOH), perfluorohexanoic acid(PFHxA), perfluoro-n-pentanoic acid (PFPeA), perfluorobutanoic acid (PFBA) and 5:3 polyfluorinated acid. At different stages of 6:2 FTOH degradation, a change of bacteria and the predominant population became somewhat different. Based on the experimental results for 100 d, at the Phylum level, the biodegradation of 6:2 FTOH greatly increases the abundance of Chloroflexi (+24.8\%) and decreases the abundance of Proteobacteria (-17.8\%) and Firmicutes (-15.9\%). At the Class level, due to the biodegradation of 6:2 FTOH, bacteria with notable increases included Anaerolineae (+19.6\%) and δ-Proteobacteria (+4.3\%), while bacteria with notable decreases included ε-Proteobacteria (-20.0\%), Clostridia (-10.1\%), Bacilli (-5.8\%) and γ-Proteobacteria (-4.2\%). At the Genus level, due to the biodegradation of 6:2 FTOH, bacteria with notable increases included Anaerolineaceae_(uncultured) (+19.1\%) and Thioalkalispira (+13.3\%), while bacteria with notable decreases included Vibrio (-14.1\%), Sulfurimonas (-13.2\%), Bacillus (-5.1\%), Sulfurovum (-4.2\%) and Fusibacter (-4.1\%). These results are helpful for predicting the response of bacteria to the contamination of polyfluoalkyl substances and isolating the bacteria capable of the biodegradation of polyfluoalkyl substances. {\textcopyright} 2017, Science Press. All right reserved.},
	keywords = {alcohol, Bacteria, Bacterial community structure, Bacteriology, bacterium, biodegradation, bioremediation, Carboxylic acids, China, Chromatography, classification, Consumer products, Degradation, Denaturing gradient gel electrophoreses (DGGE), Diversity index, Electrophoresis, ENVIRONMENTAL, Ethanol, fluorocarbon, Fluorocarbons, Fluorotelomer alcohols, Fluorotelomer carboxylic acids, Fluorotelomer unsaturated carboxylic acids, Geologic Sediments, High-throughput sequencing, Ketones, Liquid, Liquid chromatography, mass spectrometry, metabolism, microbiology, Microcosm experiments, river, Rivers, Sediment, sediments, Surficial sediments, Tandem mass spectrometry},
	issn = {02503301},
	doi = {10.13227/j.hjkx.201704297},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045697955\&amp;doi=10.13227\%2fj.hjkx.201704297\&amp;partnerID=40\&amp;md5=59cdce8c3cd9b9b331248826e38e4422},
	author = {Wang, D. and Hou, Z. and Q. Zhang and Y. Zhou and Lu, X.-X.}
}
