<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bai, Xinyi</style></author><author><style face="normal" font="default" size="100%">Zhang, Guangbao</style></author><author><style face="normal" font="default" size="100%">Libo Xu</style></author><author><style face="normal" font="default" size="100%">Kang Li</style></author><author><style face="normal" font="default" size="100%">Mengjun Zhang</style></author><author><style face="normal" font="default" size="100%">Yi Huang</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Rewiring photosynthetic carbon flow: Engineered cyanobacterial factories for sustainable carbohydrate production and carbon-negative biomanufacturing</style></title><secondary-title><style face="normal" font="default" size="100%">Bioresource Technology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2025</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.sciencedirect.com/science/article/pii/S0960852425008168</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">434</style></volume><pages><style face="normal" font="default" size="100%">132850</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Cyanobacteria are promising platforms for light-driven carbon fixation and carbohydrate biosynthesis. However, optimization strategies that focus solely on carbon allocation are insufficient to achieve substantial improvements in yield and sustainability. Here, &lt;em&gt;Synechococcus&lt;/em&gt; &lt;em&gt;elongatus&lt;/em&gt; PCC 7942 was engineered to enhance sucrose production by simultaneously increasing total carbon input and reinforcing the artificial sink. The engineered strain secreted 5.821 g L−1 sucrose, which was 27.4 times higher than the wild-type. Transcriptomic analysis revealed upregulation of abundant genes involved in carbon fixation, sucrose biosynthesis, and electron transport chains. Furthermore, a synthetic light-driven consortium was established to directly convert CO2 into value-added compounds. This system produced 323.5 mg L−1 polyhydroxybutyrate, reducing CO2 emissions by 12.4 g per g of polyhydroxybutyrate compared to conventional heterotrophic processes. These findings highlight the potential of cyanobacteria-based systems for carbon-negative biomanufacturing, demonstrating their role in advancing sustainable carbohydrate and biochemical production while exemplifying circular bioeconomy principles.</style></abstract></record></records></xml>