@article {27281,
	title = {Smoking-Related DNA Methylation is Associated with DNA Methylation Phenotypic Age Acceleration: The Veterans Affairs Normative Aging Study},
	journal = {Int J Environ Res Public Health},
	volume = {16},
	number = {13},
	year = {2019},
	note = {Yang, Yang Gao, Xu Just, Allan C Colicino, Elena Wang, Cuicui Coull, Brent A Hou, Lifang Zheng, Yinan Vokonas, Pantel Schwartz, Joel Baccarelli, Andrea A eng Switzerland Int J Environ Res Public Health. 2019 Jul 3;16(13). pii: ijerph16132356. doi: 10.3390/ijerph16132356.},
	pages = {2356},
	type = {Journal Article},
	abstract = {DNA methylation may play a critical role in aging and age-related diseases. DNA methylation phenotypic age (DNAmPhenoAge) is a new aging biomarker and predictor of chronic disease risk. While smoking is a strong risk factor for chronic diseases and influences methylation, its influence on DNAmPhenoAge is unknown. We investigated associations of self-reported and epigenetic smoking indicators with DNAmPhenoAge acceleration in a longitudinal aging study in eastern Massachusetts. DNA methylation was measured in whole blood samples from multiple visits for 692 male participants in the Veterans Affairs Normative Aging Study during 1999-2013. Acceleration was defined using residuals from linear regression of the DNAmPhenoAge on the chronological age. Cumulative smoking (pack-years) was significantly associated with DNAmPhenoAge acceleration, whereas self-reported smoking status was not. We observed significant validated associations between smoking-related loci and DNAmPhenoAge acceleration for 52 CpG sites, where 18 were hypomethylated and 34 were hypermethylated, mapped to 16 genes. The AHRR gene had the most loci (N = 8) among the 16 genes. We generated a smoking aging index based on these 52 loci, which showed positive significant associations with DNAmPhenoAge acceleration. These epigenetic biomarkers may help to predict age-related risks driven by smoking.},
	keywords = {80 and over Aging/*genetics Biomarkers CpG Islands *DNA Methylation Humans Longitudinal Studies Male Massachusetts Middle Aged Phenotype Self Report Tobacco Smoking/*genetics *Veterans *DNA methylation phenotypic age *aging acceleration *aging biomarker, Aged Aged},
	issn = {1660-4601 (Electronic) 1660-4601 (Linking)},
	doi = {10.3390/ijerph16132356},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/31277270},
	author = {Yang, Y. and Gao, X. and Just, A. C. and Colicino, E. and C. Wang and Coull, B. A. and Hou, L. and Zheng, Y. and Vokonas, P. and Schwartz, J. and Baccarelli, A. A.}
}
