@article {35399,
	title = {Genetically encoded bioorthogonal tryptophan decaging in living cells},
	journal = {Nature Chemistry},
	year = {2024},
	month = {2024/02/28},
	abstract = {Tryptophan (Trp) plays a critical role in the regulation of protein structure, interactions and functions through its π system and indole N{\textendash}H group. A generalizable method for blocking and rescuing Trp interactions would enable the gain-of-function manipulation of various Trp-containing proteins in vivo, but generating such a platform remains challenging. Here we develop a genetically encoded N1-vinyl-caged Trp capable of rapid and bioorthogonal decaging through an optimized inverse electron-demand Diels{\textendash}Alder reaction, allowing site-specific activation of Trp on a protein of interest in living cells. This chemical activation of a genetically encoded caged-tryptophan (Trp-CAGE) strategy enables precise activation of the Trp of interest underlying diverse important molecular interactions. We demonstrate the utility of Trp-CAGE across various protein families, such as catalase-peroxidases and kinases, as translation initiators and posttranslational modification readers, allowing the modulation of epigenetic signalling in a temporally controlled manner. Coupled with computer-aided prediction, our strategy paves the way for bioorthogonal Trp activation on more than 28,000 candidate proteins within their native cellular settings.},
	isbn = {1755-4349},
	author = {Zhu, Yuchao and Ding, Wenlong and Chen, Yulin and Shan, Ye and Liu, Chao and Xinyuan Fan* and Shixian Lin* and Peng R. Chen*}
}
