@article {35379,
	title = {Photocatalytic Chemical Crosslinking for Profiling RNA{\textendash}Protein Interactions in Living Cells},
	journal = {Angewandte Chemie International EditionAngewandte Chemie International Edition},
	volume = {61},
	number = {27},
	year = {2022},
	note = {https://doi.org/10.1002/anie.202202008},
	month = {2022/07/04},
	pages = {e202202008},
	publisher = {John Wiley \&amp;amp; Sons, Ltd},
	type = {https://doi.org/10.1002/anie.202202008},
	abstract = {Abstract The dynamic interactions between RNAs and proteins play crucial roles in regulating diverse cellular processes. Proteome-wide characterization of these interactions in their native cellular context remains desirable but challenging. Herein, we developed a photocatalytic crosslinking (PhotoCAX) strategy coupled with mass spectrometry (PhotoCAX-MS) and RNA sequencing (PhotoCAX-seq) for the study of the composition and dynamics of protein-RNA interactions. By integrating the blue light-triggered photocatalyst with a dual-functional RNA?protein crosslinker (RP-linker) and the phase separation-based enrichment strategy, PhotoCAX-MS revealed a total of 2044 RBPs in human HEK293 cells. We further employed PhotoCAX to investigate the dynamic change of RBPome in macrophage cells upon LPS-stimulation, as well as the identification of RBPs interacting directly with the 5? untranslated regions of SARS-CoV-2 RNA.},
	keywords = {Crosslinking, Photocatalysis, RNA-Binding Protein, RNA{\textendash}Protein Interactome, SARS-CoV-2},
	isbn = {1433-7851},
	author = {Huixin Luo and Tang, Wei and Hongyu Liu and Zeng, Xiangmei and Ngai, William Shu Ching and Gao, Rui and Li, Heyun and Li, Ran and Zheng, Huangtao and Guo, Jianting and Qin, Fangfei and Gang Wang and Li, Kexin and Xinyuan Fan* and Zou, Peng* and Peng R. Chen*}
}
