<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Qi Zhou</style></author><author><style face="normal" font="default" size="100%">Jia, Lixia</style></author><author><style face="normal" font="default" size="100%">Li, Yuanwei</style></author><author><style face="normal" font="default" size="100%">Wu, Weizhong</style></author><author><style face="normal" font="default" size="100%">Wang, Jianlong</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Significantly Enhanced Nitrate and Phosphorus Removal by Pyrite/Sawdust Composite-Driven Mixotrophic Denitrification with Boosted Electron Transfer: Comprehensive Evaluation of Water-Gas-Biofilm Phases during a Long-Term Study</style></title><secondary-title><style face="normal" font="default" size="100%">ENVIRONMENTAL SCIENCE &amp;amp; TECHNOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biofilm</style></keyword><keyword><style  face="normal" font="default" size="100%">DOM</style></keyword><keyword><style  face="normal" font="default" size="100%">Electron transfer</style></keyword><keyword><style  face="normal" font="default" size="100%">mixotrophic denitrification</style></keyword><keyword><style  face="normal" font="default" size="100%">pyrite/sawdust composites</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAY 29</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">23</style></number><publisher><style face="normal" font="default" size="100%">AMER CHEMICAL SOC</style></publisher><pub-location><style face="normal" font="default" size="100%">1155 16TH ST, NW, WASHINGTON, DC 20036 USA</style></pub-location><volume><style face="normal" font="default" size="100%">58</style></volume><pages><style face="normal" font="default" size="100%">10149-10161</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Further reducing total nitrogen (TN) and total phosphorus (TP) in the secondary effluent needs to be realized effectively and in an eco-friendly manner. Herein, four pyrite/sawdust composite-based biofilters were established to treat simulated secondary effluent for 304 days. The results demonstrated that effluent TN and TP concentrations from biofilters under the optimal hydraulic retention time (HRT) of 3.5 h were stable at &amp;lt;2.0 and 0.1 mg/L, respectively, and no significant differences were observed between inoculated sludge sources. The pyrite/sawdust composite-based biofilters had low N2O, CH4, and CO2 emissions, and the effluent&amp;#039;s DOM was mainly composed of five fluorescence components. Moreover, mixotrophic denitrifiers (Thiothrix) and sulfate-reducing bacteria (Desulfosporosinus) contributing to microbial nitrogen and sulfur cycles were enriched in the biofilm. Co-occurrence network analysis deciphered that Chlorobaculum and Desulfobacterales were key genera, which formed an obvious sulfur cycle process that strengthened the denitrification capacity. The higher abundances of genes encoding extracellular electron transport (EET) chains/mediators revealed that pyrite not only functioned as an electron conduit to stimulate direct interspecies electron transfer by flagella but also facilitated EET-associated enzymes for denitrification. This study comprehensively evaluates the water-gas-biofilm phases of pyrite/sawdust composite-based biofilters during a long-term study, providing an in-depth understanding of boosted electron transfer in pyrite-based mixotrophic denitrification systems.</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type></record></records></xml>