@article {ZHOU2019960,
	title = {The effects of graphene oxide on nitrification and N2O emission: Dose and exposure time dependent},
	journal = {Environmental Pollution},
	volume = {252},
	year = {2019},
	pages = {960 - 966},
	abstract = {With the extensive application of graphene oxide (GO), its leakage and release into wastewater treatment plants become inevitable. However, the toxicity of graphene oxide (GO) on nitrification process and the underlying mechanisms still remain unclear. In this study, the toxic effects of GO at concentration of 10 and 100~mg/L in 4~h and 10 days were evaluated with sealed reactors operated in sequencing batch mode. In the initial 4~h, both GO concentrations showed no negative effect on nitrogen conversion. However, the exposure to 100~mg/L GO significantly weakened the NH+ 4-N and NO- 2-N conversion capabilities and intensified the nitrous oxide (N2O) generation after 10 days. Extracellular polymeric substance (EPS) analysis suggested that 100 mg/L GO decreased the protein content of the nitrifying activated sludge. Moreover, reactive oxygen species (ROS) level was promoted by 100 mg/L GO owing to the impaired endogenous antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT), which caused oxidative stress to bacteria. Finally, quantitative PCR results confirmed that nitrite-oxidizing bacteria (NOB) and complete ammonia oxidizing bacteria (CAOB) were more sensitive to GO, which was the primary cause for the significant promotion of N2O generation in the high GO concentration. This study offered new insights in the toxicity of GO on nitrification and N2O generation in the terms of dose and exposure time.},
	keywords = {Comammox, Graphene oxide, Nitrification, Nitrous oxide},
	issn = {0269-7491},
	doi = {https://doi.org/10.1016/j.envpol.2019.06.009},
	url = {http://www.sciencedirect.com/science/article/pii/S0269749119309170},
	author = {Nan Zhou and Zhirong Zhao and Huihui Wang and Xiangyu Chen and Mingyuan Wang and Shishi He and Liu, Wen and Zheng, Maosheng}
}
