<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Shang, Yu</style></author><author><style face="normal" font="default" size="100%">Qian Zhou</style></author><author><style face="normal" font="default" size="100%">Wang, Tiantian</style></author><author><style face="normal" font="default" size="100%">Jiang, Yuting</style></author><author><style face="normal" font="default" size="100%">Zhong, Yufang</style></author><author><style face="normal" font="default" size="100%">Qian, Guangren</style></author><author><style face="normal" font="default" size="100%">Zhu, Tong</style></author><author><style face="normal" font="default" size="100%">Xinghua Qiu</style></author><author><style face="normal" font="default" size="100%">An, Jing</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Airborne nitro-PAHs induce Nrf2/ARE defense system against oxidative stress and promote inflammatory process by activating PI3K/Akt pathway in A549 cells</style></title><secondary-title><style face="normal" font="default" size="100%">Toxicology in Vitro</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Oct</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">44</style></volume><pages><style face="normal" font="default" size="100%">66-73</style></pages><isbn><style face="normal" font="default" size="100%">0887-2333</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Ambient particulate matter (PM) is a worldwide health issue of concern. However, limited information is available regarding the toxic contributions of the nitro-derivatives of polycyclic aromatic hydrocarbons (nitro-PAHs). This study intend to examine whether 1-nitropyrene (1-NP) and 3-nitrofluoranthene (3-NF) could activate the nuclear factor-erythroid 2-related factor 2/antioxidant response element (Nrf2/ARE) antioxidant defense system, and whether the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway participates in regulating pro-inflammatory responses in A549 cells. Firstly, 1-NP and 3-NF concentration-dependently induced cellular apoptosis, reactive oxygen species (ROS) generation, DNA damage, S phase cell cycle arrest and differential expression of related cytokine genes. Secondly, 1-NP and 3-NF activated the Nrf2/ARE defense system, as evidenced by increased protein expression levels and nuclear translocation of transcription factor Nrf2, elevated Nrf2/ARE binding activity, up-regulated expression of the target gene heme oxygenase-1 (HO-1). Significantly increased protein expression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and phosphorylation level of Akt indicated that the PI3K/Akt pathway was activated during pro-inflammatory process. Further, both PI3K inhibitor (LY294002) and Akt inhibitor (MK-2206) reversed the elevated TNF-alpha expression to control level. Our results suggested that Nrf2/ARE pathway activation might cause an initiation step in cellular protection against oxidative stress caused by nitro-PAHs, and the PI3K/Akt pathway participated in regulating inflammatory responses.</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000409395000009</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Times Cited: 017&lt;/p&gt;</style></notes></record></records></xml>