<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chengang Xiang</style></author><author><style face="normal" font="default" size="100%">Yuanyuan Du</style></author><author><style face="normal" font="default" size="100%">Gaofan Meng</style></author><author><style face="normal" font="default" size="100%">Liew Soon Yi</style></author><author><style face="normal" font="default" size="100%">Shicheng Sun</style></author><author><style face="normal" font="default" size="100%">Nan Song</style></author><author><style face="normal" font="default" size="100%">Xiaonan Zhang</style></author><author><style face="normal" font="default" size="100%">Yiwei Xiao</style></author><author><style face="normal" font="default" size="100%">Jie Wang</style></author><author><style face="normal" font="default" size="100%">Zhigang Yi</style></author><author><style face="normal" font="default" size="100%">Yifang Liu</style></author><author><style face="normal" font="default" size="100%">Bingqing Xie</style></author><author><style face="normal" font="default" size="100%">Min Wu</style></author><author><style face="normal" font="default" size="100%">Jun Shu</style></author><author><style face="normal" font="default" size="100%">Da Sun</style></author><author><style face="normal" font="default" size="100%">Jun Jia</style></author><author><style face="normal" font="default" size="100%">Zhen Liang</style></author><author><style face="normal" font="default" size="100%">Dong Sun</style></author><author><style face="normal" font="default" size="100%">Yanxiang Huang</style></author><author><style face="normal" font="default" size="100%">Yan Shi</style></author><author><style face="normal" font="default" size="100%">Jun Xu</style></author><author><style face="normal" font="default" size="100%">Fengmin Lu</style></author><author><style face="normal" font="default" size="100%">Cheng Li</style></author><author><style face="normal" font="default" size="100%">Kuanhui Xiang</style></author><author><style face="normal" font="default" size="100%">Zhenghong Yuan</style></author><author><style face="normal" font="default" size="100%">Shichun Lu</style></author><author><style face="normal" font="default" size="100%">Hongkui Deng</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Long-term functional maintenance of primary human hepatocytes in vitro</style></title><secondary-title><style face="normal" font="default" size="100%">Science</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://pubmed.ncbi.nlm.nih.gov/31023926/</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">364</style></volume><pages><style face="normal" font="default" size="100%">399-402</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.</style></abstract><issue><style face="normal" font="default" size="100%">6438</style></issue></record></records></xml>