@article {31225,
	title = {A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts},
	journal = {Cell Res },
	volume = {29},
	year = {2019},
	pages = {696-670},
	abstract = {Terminally differentiated cells can be generated by lineage reprogramming, which is, however, hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming.},
	url = {https://pubmed.ncbi.nlm.nih.gov/31270412/},
	author = {Bingqing Xie and Da Sun and Yuanyuan Du and Jun Jia and Shicheng Sun and Jun Xu and Yifang Liu and Chengang Xiang and Sitong Chen and Huangfan Xie and Qiming Wang and Guangya Li and Xuehui Lyu and Hui Shen and Shiyu Li and Min Wu and Xiaonan Zhang and Yue Pu and Kuanhui Xiang and Weifeng Lai and Peng Du and Zhenghong Yuan and Cheng Li and Cheng Li and Shichun Lu and Hongkui Deng}
}
