<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Cui, X. X.</style></author><author><style face="normal" font="default" size="100%">Gong, J. C.</style></author><author><style face="normal" font="default" size="100%">Han, H. L.</style></author><author><style face="normal" font="default" size="100%">He, L. C.</style></author><author><style face="normal" font="default" size="100%">Teng, Y. B.</style></author><author><style face="normal" font="default" size="100%">Tetley, T.</style></author><author><style face="normal" font="default" size="100%">Sinharay, R.</style></author><author><style face="normal" font="default" size="100%">Chung, K. F.</style></author><author><style face="normal" font="default" size="100%">Islam, T.</style></author><author><style face="normal" font="default" size="100%">Gilliland, F.</style></author><author><style face="normal" font="default" size="100%">Grady, S.</style></author><author><style face="normal" font="default" size="100%">Garshick, E.</style></author><author><style face="normal" font="default" size="100%">Z. Li</style></author><author><style face="normal" font="default" size="100%">Zhang, J. F.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Relationship between free and total malondialdehyde, a well-established marker of oxidative stress, in various types of human biospecimens</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Thoracic Disease</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Thorac Dis</style></alt-title><short-title><style face="normal" font="default" size="100%">J Thorac DisJ Thorac Dis</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">air-pollution</style></keyword><keyword><style  face="normal" font="default" size="100%">biological markers</style></keyword><keyword><style  face="normal" font="default" size="100%">biomarkers</style></keyword><keyword><style  face="normal" font="default" size="100%">disease</style></keyword><keyword><style  face="normal" font="default" size="100%">exhaled breath condensate</style></keyword><keyword><style  face="normal" font="default" size="100%">healthy</style></keyword><keyword><style  face="normal" font="default" size="100%">lipid-peroxidation</style></keyword><keyword><style  face="normal" font="default" size="100%">malonaldehyde</style></keyword><keyword><style  face="normal" font="default" size="100%">malondialdehyde (mda)</style></keyword><keyword><style  face="normal" font="default" size="100%">nasal fluid</style></keyword><keyword><style  face="normal" font="default" size="100%">nasal secretions</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxidative Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">saliva</style></keyword><keyword><style  face="normal" font="default" size="100%">serum</style></keyword><keyword><style  face="normal" font="default" size="100%">total plasma malondialdehyde</style></keyword><keyword><style  face="normal" font="default" size="100%">urine</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">May</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">5</style></number><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">3088-+</style></pages><isbn><style face="normal" font="default" size="100%">2072-1439</style></isbn><language><style face="normal" font="default" size="100%">English</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Background: Oxidative stress is involved in thoracic diseases and health responses to air pollution. Malondialdehyde (MDA) is a well-established marker of oxidative stress, but it may be present in unconjugated and conjugated forms. To our knowledge, no studies have conducted a systemic evaluation of both free MDA (unconjugated MDA) and total MDA (the sum of both unconjugated and conjugated MDA) across various types of human biospecimens.Methods: Free MDA and total MDA were simultaneously measured in a range of human biospecimens, including nasal fluid (N=158), saliva (N=158), exhaled breath condensate (N=40), serum (N=232), and urine (N=429). All samples were analyzed using an HPLC-fluorescence method with high sensitivity and specificity. Due to the right skewed distribution of free MDA and total MDA, we performed natural-log transformation before subsequent statistical analyses. The relationship between the natural log of free and total MDA was evaluated by R-2 of simple linear regression. T test was used for comparisons of means between two groups. One-way analysis of variance was used in combination with Tukey's test to compare the natural log of the ratio of free MDA to total MDA across various types of biospecimens.Results: For exhaled breath condensate, serum, urine, nasal fluid and saliva samples, the R-2 between free and total MDA were 0.61, 0.22, 0.59, 0.47 and 0.06, respectively; the medians of the free MDA to total MDA ratio were 48.1%, 17.4%, 9.8%, 5.1% and 3.0%, respectively; the free MDA to total MDA ratio in EBC &amp;gt; serum &amp;gt; urine &amp;gt; nasal fluid &amp;gt; saliva (P &amp;lt; 0.001 for pairwise comparisons).Conclusions: For exhaled breath condensate and urine samples, using either free or total MDA can provide information regarding the level of oxidative stress; however, that is not the case for serum, nasal fluid, and saliva given the low correlations between free and total MDA.&lt;/p&gt;</style></abstract><accession-num><style face="normal" font="default" size="100%">WOS:000435834200087</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Gk0wzTimes Cited:0Cited References Count:51&lt;/p&gt;</style></notes><auth-address><style face="normal" font="default" size="100%">Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USAPeking Univ, Coll Environm Sci &amp; Engn, BIC ESAT, Beijing 100871, Peoples R ChinaPeking Univ, Coll Environm Sci &amp; Engn, SKL ESPC, Beijing 100871, Peoples R ChinaDuke Univ, Duke Global Hlth Inst, Durham, NC USADuke Kunshan Univ, Kunshan 215316, Peoples R ChinaImperial Coll London, Natl Heart &amp; Lung Inst, London, EnglandUniv Southern Calif, Keck Sch Med, Dept Prevent Med, Los Angeles, CA 90033 USAVet Affairs Boston Healthcare Syst, Res &amp; Dev Serv, Boston, MA USAHarvard Med Sch, Boston, MA USAVA Boston Healthcare Syst, Med Serv, Pulm Allergy Sleep &amp; Crit Care Med Sect, Boston, MA USABrigham &amp; Womens Hosp, Dept Med, Channing Div Network Med, 75 Francis St, Boston, MA 02115 USAShanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Pediat, Shanghai 201620, Peoples R China</style></auth-address></record></records></xml>