@article {doi:10.1021/es102299j,
	title = {Viability and Metal Reduction of Shewanella oneidensis MR-1 under CO2 Stress: Implications for Ecological Effects of CO2 Leakage from Geologic CO2 Sequestration},
	journal = {Environmental Science \&amp;amp; Technology},
	volume = {44},
	number = {23},
	year = {2010},
	note = {PMID: 21058700},
	pages = {9213-9218},
	abstract = {To study potential ecological impacts of CO2 leakage to shallow groundwater and soil/sediments from geologic CO2 sequestration (GCS) sites, this work investigated the viability and metal reduction of Shewanella oneidensis MR-1 under CO2 stress. While MR-1 could grow under high-pressure nitrogen gas (500 psi), the mix of 1\% CO2 with N2 at total pressures of 15 or 150 psi significantly suppressed the growth of MR-1, compared to the N2 control. When CO2 partial pressures were over 15 psi, the growth of MR-1 stopped. The reduced bacterial viability was consistent with the pH decrease and cellular membrane damage under high pressure CO2. After exposure to 150 psi CO2 for 5 h, no viable cells survived, the cellular contents were released, and microscopy images confirmed significant cell structure deformation. However, after a relatively short exposure (25 min) to 150 psi CO2, MR-1 could fully recover their growth within 24 h after the stress was removed, and the reduction of MnO2 by MR-1 was observed right after the stress was removed. Furthermore, MR-1 survived better if the cells were aggregated rather than suspended, or if pH buffering minerals, such as calcite, were present. To predict the cell viability under different CO2 pressures and exposure times, a two-parameter mathematical model was developed.},
	doi = {10.1021/es102299j},
	url = {https://doi.org/10.1021/es102299j},
	author = {Wu, Bing and Shao, Hongbo and Wang, Zhipeng and Yandi Hu and Tang, Yinjie J. and Jun, Young-Shin*}
}
